Journal: Frontiers in Immunology

Article Title: The Culture Dish Surface Influences the Phenotype and Cytokine Production of Human Monocyte-Derived Dendritic Cells

doi: 10.3389/fimmu.2019.02352

Figure Lengend Snippet: Influence of the culture dish on the phenotype of differentially stimulated moDC. The phenotype was analyzed by flow cytometry using the indicated surface molecules. % positive cells or median fluorescence intensity (MFI) are shown. Color code: Control (DMSO)—green, immunogenic DC populations (LPS & Jonuleit cytokine cocktail)—red, tolerogenic DC populations (Dexamethasone with vitamin D3 & IL-10)—blue. Squares (left) : standard culture dish; circles (right) : non-adherent culture dish. The median is marked with a line. Significance values are given in grades * P < 0.05, ** P < 0.01, and *** P < 0.001. Black significance grades are the result of a collective 2 way ANOVA testing for all treatments, red grades are from separate tests for the iDC/tolDC and immunogen subgroups ( n = 8).

Article Snippet: LPS (100 ng/ml; Sigma-Aldrich, Taufkirchen, Germany) and the Jonuleit cytokine cocktail [10 ng/ml of IL-1β, 10 ng/ml of TNF, 1,000 U/ml of IL-6; all ImmunoTools, Friesoythe, Germany, and 1 μg/ml of prostaglandin E2 (PGE 2 ); SigmaAldrich, Taufkirchen, Germany] were added 24 h before harvesting, respectively.

Techniques: Flow Cytometry, Fluorescence, Control

Journal: Frontiers in Immunology

Article Title: The Culture Dish Surface Influences the Phenotype and Cytokine Production of Human Monocyte-Derived Dendritic Cells

doi: 10.3389/fimmu.2019.02352

Figure Lengend Snippet: Influence of the culture dish on the cytokine production of differentially stimulated moDC. Cytokines were measured in cell free culture supernatants of the generated moDC populations. Color code: Control (DMSO)—green, immunogenic DC populations (LPS and Jonuleit cytokine cocktail, respectively)—red, tolerogenic DC populations (Dexamethasone with vitamin D3 and IL-10, respectively)—blue. Circles (left) : standard culture dish; squares (right) : non-adherent culture dish. The median is marked with a line. Significance values are given in grades * P < 0.05, ** P < 0.01, and *** P < 0.001. Black significance grades are the result of a collective 2 way ANOVA testing for all treatments, red grades are from separate tests for the iDC/tolDC and immunogen subgroups ( n = 6).

Article Snippet: LPS (100 ng/ml; Sigma-Aldrich, Taufkirchen, Germany) and the Jonuleit cytokine cocktail [10 ng/ml of IL-1β, 10 ng/ml of TNF, 1,000 U/ml of IL-6; all ImmunoTools, Friesoythe, Germany, and 1 μg/ml of prostaglandin E2 (PGE 2 ); SigmaAldrich, Taufkirchen, Germany] were added 24 h before harvesting, respectively.

Techniques: Generated, Control

Journal: Frontiers in Immunology

Article Title: The Culture Dish Surface Influences the Phenotype and Cytokine Production of Human Monocyte-Derived Dendritic Cells

doi: 10.3389/fimmu.2019.02352

Figure Lengend Snippet: Antigen-specific T cell induction is not influenced by the culture dish conditions of the moDC. Autologous PBMC depleted of monocytes were co-cultured with PPD-loaded DC generated on the indicated surface and matured with indicated stimuli for 7 days, and antigen-specific IFN-γ secretion by CD4+ and CD8+ cells was analyzed by flow cytometry with LPS-DC as stimulators (± PPD). Delta LPS: moDC cultured on a conventional culture dish, stimulated with LPS; Hydrocell LPS: moDC cultured on a non-adherent culture dish, stimulated with LPS; Delta Jonuleit: moDC cultured on a conventional culture dish, stimulated with Jonuleit cytokine cocktail (TNF, IL-6, IL-1β, and PGE 2 ); Hydrocell Jonuleit: moDC cultured on a non-adherent culture dish, stimulated with Jonuleit cytokine cocktail. Each color-coded symbol represents results from one individual ( n = 9).

Article Snippet: LPS (100 ng/ml; Sigma-Aldrich, Taufkirchen, Germany) and the Jonuleit cytokine cocktail [10 ng/ml of IL-1β, 10 ng/ml of TNF, 1,000 U/ml of IL-6; all ImmunoTools, Friesoythe, Germany, and 1 μg/ml of prostaglandin E2 (PGE 2 ); SigmaAldrich, Taufkirchen, Germany] were added 24 h before harvesting, respectively.

Techniques: Cell Culture, Generated, Flow Cytometry